Question 21
Food Substances and EnzymesIn a Biuret test, some protein was mixed with sodium hydroxide solution. Which of the following chemicals should be added to the mixture for a positive result?
- A.Mercurous nitrate
- B.Copper sulphate
- C.Mercuric nitrate
- D.Sodium carbonate
- E.Ammonium hydroxide.
Show the answer and explanation
Answer: B (Copper sulphate)
Explanation
In the Biuret test a few drops of dilute copper sulphate are added to the food and sodium hydroxide; a violet colour shows protein.