JAMB Biology 1984 Question 21

Question 21

Food Substances and Enzymes

In a Biuret test, some protein was mixed with sodium hydroxide solution. Which of the following chemicals should be added to the mixture for a positive result?

  1. A.Mercurous nitrate
  2. B.Copper sulphate
  3. C.Mercuric nitrate
  4. D.Sodium carbonate
  5. E.Ammonium hydroxide.
Show the answer and explanation

Answer: B (Copper sulphate)

Explanation

In the Biuret test a few drops of dilute copper sulphate are added to the food and sodium hydroxide; a violet colour shows protein.